CRM External Seminar: Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics, Germany Dr Anne Grapin-Botton Managing Director, Max Planck Institute of Molecular Cell Biology and Genetics Contact details Email: botton@mpi-cbg.de Talk title Self-organisation in pancreas organoids: from complex ductal networks to differentiation Host Student-led Abstract To understand pancreas development, as a complement to in vivo investigations, Anne and her team, designed simplified in vitro systems that can be monitored and manipulated more extensively than the whole embryo. They established three-dimensional culture conditions that enable the efficient expansion, differentiation, and morphogenesis of pancreatic progenitors isolated from mouse embryos, human fetuses or produced from human pluripotent stem cells. The mouse system is architecturally the most elaborate, encompassing all epithelial pancreatic cell types spatially arranged similarly to the developing organ in vivo. Notably, acinar cells, the cells that produce digestive enzymes, are found at the tip of emerging branches and a network or ducts connects them, though lacking the outlet found in vivo. Endocrine cells form a small percentage interspersed between ducts. They investigated the mechanisms leading to organoid self-organization, notably lumen formation, branching, differentiation and collective movements. Starting from the onset of organoid formation we observe a first step of epithelial cell aggregation and compaction which is necessary to enable growth. Under some conditions, collective movements, which are not observed in single cells, emerge from the collective. The talk will start with an introduction of the systems and present some examples of their use to study quantitatively the dynamics of endocrine cell differentiation in human (quantitative live imaging, human mutations), how a network of ducts with narrow elongated lumens forms rather than single spherical lumens found in many organoids and the establishment of a 3D high content screen on organoids. Bio Anne has a background in developmental biology and initially studied nervous system (College de France, Paris, Le Douarin lab) and endoderm (Harvard University, Melton lab) development. She started her independent career at the Swiss Institute for Experimental Cancer Research (Lausanne, Switzerland) and subsequently at the Ecole Polytechnique Federale in Lausanne, at the Danish Stem Cell Center (DanStem, Copenhagen, Denmark) and Max Planck Institute of Molecular Cell Biology and Genetics (Dresden, Germany). Her laboratory is currently focusing on pancreas development with the overall goals of understanding how pancreatic cells differentiate during embryogenesis, and determining what limits the pancreatic cells’ regeneration in adults. More specifically, Professor Grapin-Botton and her group investigate the impact of the cellular and organ architecture on the cells’ fate choices and the dynamics of decision processes. Aug 31 2023 12.00 - 13.00 CRM External Seminar: Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics, Germany Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics This event is a Centre for Regenerative Medicine (CRM) external seminar which will take place in the seminar room, 1st floor, IRR North. Where to find us
CRM External Seminar: Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics, Germany Dr Anne Grapin-Botton Managing Director, Max Planck Institute of Molecular Cell Biology and Genetics Contact details Email: botton@mpi-cbg.de Talk title Self-organisation in pancreas organoids: from complex ductal networks to differentiation Host Student-led Abstract To understand pancreas development, as a complement to in vivo investigations, Anne and her team, designed simplified in vitro systems that can be monitored and manipulated more extensively than the whole embryo. They established three-dimensional culture conditions that enable the efficient expansion, differentiation, and morphogenesis of pancreatic progenitors isolated from mouse embryos, human fetuses or produced from human pluripotent stem cells. The mouse system is architecturally the most elaborate, encompassing all epithelial pancreatic cell types spatially arranged similarly to the developing organ in vivo. Notably, acinar cells, the cells that produce digestive enzymes, are found at the tip of emerging branches and a network or ducts connects them, though lacking the outlet found in vivo. Endocrine cells form a small percentage interspersed between ducts. They investigated the mechanisms leading to organoid self-organization, notably lumen formation, branching, differentiation and collective movements. Starting from the onset of organoid formation we observe a first step of epithelial cell aggregation and compaction which is necessary to enable growth. Under some conditions, collective movements, which are not observed in single cells, emerge from the collective. The talk will start with an introduction of the systems and present some examples of their use to study quantitatively the dynamics of endocrine cell differentiation in human (quantitative live imaging, human mutations), how a network of ducts with narrow elongated lumens forms rather than single spherical lumens found in many organoids and the establishment of a 3D high content screen on organoids. Bio Anne has a background in developmental biology and initially studied nervous system (College de France, Paris, Le Douarin lab) and endoderm (Harvard University, Melton lab) development. She started her independent career at the Swiss Institute for Experimental Cancer Research (Lausanne, Switzerland) and subsequently at the Ecole Polytechnique Federale in Lausanne, at the Danish Stem Cell Center (DanStem, Copenhagen, Denmark) and Max Planck Institute of Molecular Cell Biology and Genetics (Dresden, Germany). Her laboratory is currently focusing on pancreas development with the overall goals of understanding how pancreatic cells differentiate during embryogenesis, and determining what limits the pancreatic cells’ regeneration in adults. More specifically, Professor Grapin-Botton and her group investigate the impact of the cellular and organ architecture on the cells’ fate choices and the dynamics of decision processes. Aug 31 2023 12.00 - 13.00 CRM External Seminar: Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics, Germany Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics This event is a Centre for Regenerative Medicine (CRM) external seminar which will take place in the seminar room, 1st floor, IRR North. Where to find us
Aug 31 2023 12.00 - 13.00 CRM External Seminar: Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics, Germany Dr Anne Grapin-Botton, Max Planck Institute of Molecular Cell Biology and Genetics
