Location, cost, access and specifications of the Leica TCS SP8 confocal microscope with 5 detectors. Introduction Location: CRM ground floor, room G.43 Access (after training): 24/24h, 7/7 days Cost (CRM users): £50 peak hours (8am-5pm, week days), £37.50 off-peak, time-lapse session £250 per 24h. Document Leica TCS SP8 Confocal Microscope Guide (3.57 MB / PDF) Book a microscope via PPMS Specifications Main features Inverted microscope Lasers: 405, 458/476/488/496/514, 561, 594, 633 nm Filter-free spectral detection 400-800 nm Fast resonant scanner (8kHz) complemented with Lightning for small pixel size 2 HyD detectors (45% QE @ 500 nm) and 3 PMT (30% QE @ 500 nm) for fluorescence imaging, 1 PMT for transmitted light Fully motorised XY stage for multi-positions, tilling, mosaic … On request: galvanometer stage for Z-focus (range 1500 µm, minimum step size 20 nm) Environmental chamber (temperature and 5% CO2) Software-controlled water pump for water immersion objectives Adaptive Focus Control (AFC) to correct for focus drift Advanced imaging techniques: photo-activation, time-lapse, high-content imaging (MatrixScreener module) Navigator option 3D visualisation module Leica website Objectives (More objectives available upon request at the time of booking) 10x, NA 0.40, Air 20x, NA 0.75, Air 25x, NA 0.95, Water (automatic water immersion) 40x, NA 1.1, Water (automatic water immersion) 40x, NA 1.25, Oil 63x, NA 1.4, Oil Also available: 20x, NA 0.7, multi-immersion (Water, Glycerol, Oil) Sample carriers Slides or chambered-slides Petri dishes (35 mm, 58 mm) Multi-well plates This article was published on 2024-02-26
