Location, cost, access and specifications of the Widefield Zeiss observer microscope. Introduction Location: CRM ground floor, room G.45 Access (after training): 24h, 7/7 days Cost (CRM users): £24 peak hours (8am-5pm, week days), £18 off-peak, time-lapse session £120 A guide to use this microscope Book a microscope via PPMS Specifications Main features Widefield microscope dedicated to live imaging and deconvolution Inverted microscope Zeiss Colibri7 LED light source ( LEDs: 385, 475, 555, 590, 630 nm) Filter cubes: DAPI, GFP, DsRed, mPlum and Cy5 Monochrome Hamamatsu flash 4.0 v3 camera Colour Zeiss MRc camera Motorised XY stage (multi-positions, mosaic) Motorised Z-focus Pecon incubation chamber with temperature and CO2 controllers (on request) Filter cubes DAPI (365/65, 395, 480/40) GFP (470/40, 495, 525/50) dsRed (550/25, 570, 605/70) mPlum (587/25, 605, 647/70) Cy5 (620/60, 660, 700/75) Objectives 5 x/0.1 10x/0.45 LWD 20x/ 0.5 (for multi-well plates) LWD 40x/0.75 (for multi-well plates) Plan-Apo 20x/0.8 Plan-Apo 40x/1 OIL Plan-apo 63x/1.4 OIL Available on request LWD 40x/0.75 (for multi-well plates) C-Apo 40x/1.2 Water Sample carriers Slides or chambered-slides Petri dishes (35 mm, 50 mm, 60 mm) Multi-well plates This article was published on 2024-02-26
