Location, cost, access and specifications of the Leica TCS SP8 confocal microscope with 4 detectors and Digital LightSheet (DLS). Introduction Location: CRM ground floor, room G.43 Access (after training): 24/24h, 7/7 days Cost (CRM users): £49 peak hours (8am-5pm, week days), £36.75 off-peak, time-lapse session £245 Guide to use the Leica TCS SP8 Confocal Microscope 4d DLS Book a microscope via PPMS Specifications Main features Inverted microscope Solid stateLasers: 405, 488, 552 and 638 nm Filter-free spectral detection 400-800 nm 2 HyD detectors (45% QE @ 500 nm) and 2 PMT (30% QE @ 500 nm) for fluorescence imaging, 1 PMT for transmitted light Fully motorised XY stage for multi-positions, tilling, navigator option Galvanometer stage for Z-focus (range 1500 µm, minimum step size 20 nm) Digital LightSheet module Objectives (confocal) Obj. HC PL APO 10x/0.40 CS2 Obj. HC PL APO 20x/0.75 CS2 Obj. HC PL APO 40x/1.30 Oil CS2 Obj. HC PL APO 63x/1.40 OIL CS2 Digital LightSheet Illumination objectives L 1.6x/0.05 DLS Obj. HC PL FLUOTAR 2.5x/0.07 HC PL FLUOTAR 5x/0.15 Digital LightSheet TwinFlect mirror DLS TwinFlect 2.5mm DLS TwinFlect 5mm DLS TwinFlect 7.8mm W DLS TwinFlect 7.8mm Gly Digital LightSheet Collection objectives HC PL FLUOTAR 5x/0.15 IMM DLS Obj. HC APO L10x/0.30 W DLS Obj. HC FLUOTAR L25x/0.95 W DLS Digital LightSheet Emission Filters Filter DLS BandPass 455-495 Filter DLS BandPass 504-545 Filter DLS BandPass 575-635 Filter DLS 405/488/561/633 (BandPass 422-474nm, 503-545nm, 582-615nm, 662-750nm) Filter DLS 405/488/552/638 (BandPass 420-475nm, 500-540nm, 565-620nm, 655-750nm) Filter DLS 458/514 (BandPass 470-500nm, 530-650nm) Filter DLS 488/561 (BandPass 504-544nm, 578-707nm Sample carriers Slides or chambered-slides Petri dishes (35 mm, 50 mm) Multi-well plates This article was published on 2024-02-26
