Location, cost, access and specifications of the Wiedfield Nikon Tie. Introduction Location: CRM ground floor, room G.43 Access (after training): 24h, 7/7 days Cost (CRM users): £24 peak hours (8am-5pm, week days), £18 off-peak, time-lapse session £120 A basic guide to use this microscope Book a microscope via PPMS Specifications Main features Widefield microscope dedicated to live imaging and deconvolution Nikon Eclipse Ti Inverted microscope Filter cubes: DAPI, GFP, TxRed, G2-A, Cy5 Monochrome Hamamatsu Flash4 camera Motorised XY stage (multi-positions, mosaic) Motorised Z-Focus Perfect focus Sola light engine OKO Incubation chamber with temperature and CO2 controllers JOBS module for automated imaging Objectives Plan Fluor 10x/0.3 S Plan Fluor 20x/0.5 Long Working Distance Plan Apo 20x/0.8 S Plan Fluor 40x/0.6 LWD W Apo 40x/1.15 Water (on request) Plan Apo 60x/1.40 Oil PlanApo 100x/1.40 Oil (on request) Sample carriers Slides or chambered slides Petri dishes (35mm, 50mm, 60mm) Multiwell plates This article was published on 2024-02-26